FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n02_cDNA_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n02_cDNA_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117072457
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13155571.1237117881620953No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3658450.31249450927642186No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1927540.16464504541832586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1888700.036.2184221
AGCAGTG2050300.033.3278472
ATCAACG2124300.031.88937811
TCAACGC2166800.031.24263612
TGGTATC2186600.030.9884937
GTATCAA2187550.030.925659
CAACGCA2192200.030.9169413
TATCAAC2210600.030.67800310
AACGCAG2223150.030.55597914
GGTATCA2225850.030.476948
ACGCAGA2236600.030.31024415
AGAGTAC2255250.029.92488919
CAGTGGT2281200.029.8526444
AGTGGTA2292250.029.7014875
CGCAGAG2319500.029.34293216
GTGGTAT2330350.029.1570386
GCAGAGT2359650.028.70332317
GAGTACT2345700.028.48437320
GTACTTT2330650.028.4450622
CAGAGTA2388650.028.42588618
Produced by FastQC (version 0.11.5)