FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n02_cDNA_c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n02_cDNA_c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80733678
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9392671.1634141082981504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2192580.2715818298281914No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1386360.17172015871740665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1330150.036.32431
AGCAGTG1445100.033.3645822
ATCAACG1499500.031.91052411
TCAACGC1529700.031.27194612
TGGTATC1543500.030.9680047
GTATCAA1545650.030.9349
CAACGCA1549950.030.92040413
TATCAAC1560250.030.72915810
AACGCAG1566500.030.63305914
GGTATCA1569300.030.5148148
ACGCAGA1574100.030.43967415
AGAGTAC1587800.030.0103519
CAGTGGT1612000.029.8370484
AGTGGTA1617650.029.6975755
CGCAGAG1628200.029.54467816
GTGGTAT1641600.029.180666
GCAGAGT1652450.028.97017317
CAGAGTA1676900.028.58623318
GTACTTT1644150.028.56177722
GAGTACT1655700.028.52887520
Produced by FastQC (version 0.11.5)