FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n02_cDNA_b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n02_cDNA_b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106335057
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12432021.1691365341535482No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3082170.2898545490975756No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1832300.17231382120761923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1764950.036.325921
AGCAGTG1907200.033.5718232
ATCAACG1986950.031.961611
TCAACGC2023950.031.36744112
TGGTATC2043050.031.1103367
CAACGCA2045100.031.08076913
GTATCAA2043450.031.0788299
TATCAAC2065500.030.81860510
AACGCAG2073050.030.73824314
GGTATCA2081700.030.5497828
ACGCAGA2081050.030.53550315
AGAGTAC2102500.030.09211319
CAGTGGT2132850.029.9418664
AGTGGTA2143650.029.7995685
CGCAGAG2153750.029.60330216
GTGGTAT2170250.029.3590566
GCAGAGT2193400.028.95498817
GTACTTT2173550.028.62047422
GAGTACT2190300.028.6139120
CAGAGTA2231550.028.53019518
Produced by FastQC (version 0.11.5)