FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n02_cDNA_a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n02_cDNA_a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91495083
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10920361.1935461056415457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2467350.26967022916411804No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1596480.17448806511274492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1541000.036.4684451
AGCAGTG1673400.033.5445062
ATCAACG1746150.031.93889611
TCAACGC1780700.031.28832612
GTATCAA1798400.031.0072739
TGGTATC1801250.030.9818297
CAACGCA1804250.030.95113
TATCAAC1815250.030.78496610
AACGCAG1823650.030.64715214
GGTATCA1829300.030.5334428
ACGCAGA1833450.030.42178315
CAGTGGT1861350.030.0670194
AGAGTAC1850050.030.04871219
AGTGGTA1876150.029.851085
CGCAGAG1891250.029.57723416
GTGGTAT1905150.029.3201486
GCAGAGT1923200.028.98531717
GAGTACT1917200.028.72573320
GTACTTT1914400.028.56631322
CAGAGTA1959300.028.51452818
Produced by FastQC (version 0.11.5)