FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n02_HTO2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n02_HTO2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15208430
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG893750.587667497565495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACTA3954600.043.751833
TCACATC3339150.043.7368283
TATCACA3347200.043.7324371
TTACTAT3964900.043.7080044
AGCTTAC3950200.043.7014471
GCTTACT3945300.043.7010652
TCGATAA1865800.043.7006231
CATCGGT3351750.043.687716
ATCGGTC3342900.043.6614077
ATCACAT3360100.043.6601752
TACTATC3947300.043.6527065
TCGGTCC3324800.043.637568
CGGTCCC3316450.043.6221669
TATCCCC3893700.043.610158
GGTCCCA3325800.043.5689810
ACTATCC3936500.043.5532466
GAGGCTG2198050.043.543851
CTATCCC3907000.043.539097
ATCCCCC3873050.043.537369
CACATCG3352400.043.5148774