FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n02_ADT2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n02_ADT2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25317675
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1287290.5084550615330989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCT3153950.043.8298341
TAACCAG6330850.043.761212
TTAACCA6311400.043.7483981
TGTCCGA9414600.043.7374276
GTCCGAT9388500.043.6992387
GATTCCC9384200.043.6987411
TCCGATT9387100.043.69798
GCAGTTG9514800.043.6977421
AGTTGTC9483150.043.6959883
GTTGTCC9406700.043.6905254
TTGTCCG9400550.043.687765
ACCAGTT6314750.043.6862224
ATTCCCC9308550.043.66349812
GTACCCC6259050.043.66338711
TACCCCC6225450.043.6421412
TTCCCCA9315000.043.6274513
CCGATTC9356450.043.624289
CCAGTTG6344900.043.6112635
TCTGTCA3138850.043.603495
TTCTGTC3155000.043.5981984