FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32613213
Sequences flagged as poor quality0
Sequence length27
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGC1797640.5511999078410337No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGG807080.24747025078455165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC194400.019.98211721
TTGAAAA48350.017.4360918
GGGAATG30300.016.62441
GACCTGG26450.016.4599191
GGAACTT34400.016.1409281
CTCGAAA29550.016.0498921
CCTTACG30750.016.004931
CTCGAGG37250.015.9505341
CATCGAA47450.015.9126051
CTGATCC42650.015.8049131
GACTAAC32000.015.7412281
TAGCCGG35250.015.6920511
GGGACCT24800.015.6892821
AGGGTGA40050.015.6756271
AGCTAAC60250.015.613318
CGGACGT32050.015.5854251
ATTGGAC34500.015.5151
GTAACTG29400.015.487941
CGATTGA32300.015.399681
TCGCCTG22650.015.3891258
Produced by FastQC (version 0.11.5)