FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n01_cDNA_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n01_cDNA_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117072457
Sequences flagged as poor quality0
Sequence length27
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA454500.018.1298338
ACTGATG317600.017.6708351
GACTAAC306550.017.5539721
GATGCTA322300.017.540251
GGGAATG326700.017.422981
ACTGAAC283150.017.3612771
GAATGAA370200.017.3278181
CTCGAAA296550.017.2143541
GGTATTG406800.017.168321
CTGCCTA379200.017.0689091
CGATTGA330000.017.0673161
TGCTACC329850.017.0655231
AAACCTG330950.016.7326761
GGATTAC303150.016.7217661
CGGACTG241200.016.6922131
CCTTACG340850.016.6842751
CATCGAA422700.016.6716251
TGACTAG307100.016.622981
GGAACTT365950.016.6019441
GATGAAA273650.016.4861811
Produced by FastQC (version 0.11.5)