FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n01_cDNA_c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n01_cDNA_c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80733678
Sequences flagged as poor quality0
Sequence length27
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA319200.018.0979738
ACTGATG227350.017.7185881
GACTAAC212950.017.6342531
ACTGAAC196400.017.419471
GATGCTA224950.017.2725721
CTCGAAA203450.017.2650261
GGGAATG232200.017.2218321
GAATGAA251400.017.1391411
CTGCCTA256500.017.1341671
CGATTGA222450.017.112541
GGTATTG285050.017.0836661
TGCTACC229600.017.0783061
CATCGAA288600.016.9608751
CGGACTG163600.016.879751
GGCGACT210950.016.8354341
AAACCTG219800.016.8314041
CCTTACG236100.016.7905371
GGATTAC213300.016.7484441
AGCTTGA205750.016.6431851
GGGACCT195700.016.5585781
Produced by FastQC (version 0.11.5)