FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n01_cDNA_b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n01_cDNA_b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106335057
Sequences flagged as poor quality0
Sequence length27
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA421600.018.1300058
GACTAAC274600.017.6307011
GATGCTA294600.017.5854591
ACTGATG294550.017.517121
CTCGAAA262700.017.4017221
GAATGAA337550.017.3394951
ACTGAAC260800.017.3110031
GGTATTG363950.017.1929051
GGGAATG301200.017.1826841
CGATTGA302450.016.9762211
CATCGAA376700.016.8898351
GGATTAC280350.016.8794251
CTGCCTA347100.016.8745271
TGCTACC307000.016.8067361
AAACCTG297100.016.8046151
GGAACTT343350.016.7345391
CGGACTG225700.016.7173791
ATAGACC242350.016.7131161
TGACTAG279900.016.692651
CCTTACG309400.016.5880991
Produced by FastQC (version 0.11.5)