FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n01_cDNA_a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n01_cDNA_a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91495083
Sequences flagged as poor quality0
Sequence length27
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA360000.018.0300278
GACTAAC240150.017.5967391
ACTGATG257450.017.4914321
GATGCTA259500.017.4868341
ACTGAAC221200.017.3946861
GGGAATG261300.017.1814541
GAATGAA287950.017.1270791
GGATTAC240600.017.0617561
TGCTACC259050.017.01441
CTCGAAA226600.017.012611
CGGACTG190600.016.9853521
GGTATTG314600.016.9683951
CGATTGA254950.016.9542921
CTGCCTA293750.016.9176431
CATCGAA327200.016.9024581
TGACTAG239650.016.8620191
CCTTACG256200.016.769071
AAACCTG256300.016.7010521
GGCGACT236400.016.6272751
CTAATGG254100.016.6265531
Produced by FastQC (version 0.11.5)