FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n01_HTO2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n01_HTO2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15208430
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTTC826250.020.5973955
TGTAGTT829700.020.5876164
GATGTAG837350.020.5312022
TAGTTCA837550.020.3421346
TAAGCTA269700.020.3241356
CGATGTA863700.020.3150981
TTCATGG842450.020.3099129
ATGTAGT850950.020.2746433
AAGCTAA274700.020.2533287
TCATGGT855050.020.01709610
GTTCATG858450.019.945858
AGCTAAC334500.019.9128728
CTAACAA300050.019.85168610
ACGTTAA274400.019.8457782
CATGGTG232700.019.67741211
GACGTTA312500.019.655891
GCTAACA302250.019.644349
TTAAGCT277050.019.6105335
AGTTCAT875150.019.4893957
GTGTACA132850.019.2073426
Produced by FastQC (version 0.11.5)