FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n01_GO2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n01_GO2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42317428
Sequences flagged as poor quality0
Sequence length27
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTGA219750.019.3784621
TACACGA271800.019.3080041
ACTGAAC156200.019.0044611
CTAATGG186300.018.488131
CTCACAC212400.018.441761
GGCGACT157550.018.2882371
AATTGGA158350.018.2077810
TATTGAG121450.018.1308673
GGCTCGA148900.018.080831
CCTTCGA222250.018.0781561
CATCGAA194900.018.0603871
GATGCTA118150.018.0479051
AGAATTG141800.018.0223628
GGTGACA135750.017.9691283
GGTATTG167900.017.9053731
CCGAACA112300.017.878283
ATAACGC181200.017.8490851
GTGACAC146500.017.7472974
GTGATAA135350.017.7408838
AGCTGGG183200.017.7228362
Produced by FastQC (version 0.11.5)