FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n01_ADT2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n01_ADT2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25317675
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCTA280950.019.921576
AAGCTAA290350.019.8667987
CTGATCC239050.019.8621441
AGCTAAC356750.019.7331648
AGAAACC141150.019.3994168
TGTACGG181300.019.3893910
CTAACAA303250.019.38937210
GCTAACA305150.019.2477349
ACGTTAA289700.019.2253952
GTGTACG183850.019.1316179
TGCTACC97300.019.0225831
ACTGAAC120100.019.0061051
GACGTTA339050.018.9394361
CGTTAAG294650.018.8845983
CCATGAG233600.018.868629
GGCATAG179100.018.8087123
TTCGTCC207200.018.7615094
TTGAACG109800.018.7512451
AGGCATA179950.018.7315392
CATGAGT235350.018.72411310
Produced by FastQC (version 0.11.5)