FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n02_asyn-s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n02_asyn-s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4882630
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3238886.63347417273068No Hit
CTAATAAGGCAGGTTCGTATTGTAGGTATATTTAATATAATCAGCAACCAA139060.28480552489129835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTTCG20550.034.92436611
AGGCAGG23550.030.7626367
GTTCGTA23850.030.46908213
GCAACCA23300.029.54742444
CAGGTTC24250.029.5032310
GGCAGGT24900.028.8234188
TAATCAG24450.028.70919438
AGCAACC24400.028.39978443
TAAGGCA25600.028.3882715
CCTCAAC15750.027.85475213
GCGGCGC8600.027.5108361
GCGGGTC4500.027.039221
GGTTCGT26750.026.99796112
ATAAGGC26950.026.9670544
TCGTATT27400.026.93199515
TTGTAGG27500.026.58917220
GTCGCCG10050.026.41618345
GCAGGTT27600.026.0037359
GGTCGCC9950.026.00332644
CTCCTCA16550.025.69292411
Produced by FastQC (version 0.11.2)