FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n02_asyn-s1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n02_asyn-s1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8944533
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG271140.30313488697509416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGC12450.026.0580461
GCGGGAC14100.025.0858781
GCGGGTC10300.024.2792071
GCGGGAA34050.024.0842461
GCGGGAT23450.024.0185031
GCGGGTA20600.023.8417451
GCGGGTT18000.023.7809851
GCGGGAG23800.023.759951
GCGGGTG18600.023.6194841
CTCGGTG18700.021.89656344
GCGGCGC12000.021.2151431
CGGGGCT10900.020.8627452
GCGGGGA15750.020.1691381
ATCTCGG21700.018.55856342
AGGGGAC17600.018.433111
TCTCGGT22900.018.2735843
CGGGACG4650.017.915352
AGGGGAG31000.017.2967321
GCGGGGT12800.017.2490371
CGGGTGT8900.016.9496732
Produced by FastQC (version 0.11.2)