FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n02_asyn-l2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n02_asyn-l2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4049637
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2700346.668103832516346No Hit
CTAATAAGGCAGGTTCGTATTGTAGGTATATTTAATATAATCAGCAACCAA109360.2700488957405318No Hit
GCGGCGCTACCATGAGAAATGGGTGAATGTTGAGATAATTGTTGGGATTCC62270.1537668684872249No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG46080.11378797655197243Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCGC16250.037.7135851
CCTCAAC21350.034.88021513
GCGGGGC11050.034.8671231
CTCCTCA22650.032.7793311
AGGTTCG17500.032.2693411
CTCGGTG34250.031.1366744
AGACTCC12250.031.03880744
GTCGCCG9500.030.55065345
CGGCGTT8850.030.296051
CCTCCTC25200.029.73021710
AGGCAGG18850.029.7191947
ATCTCGG36100.029.66566742
GTTCGTA19100.029.5657813
GGTCGCC9700.029.45685444
GGCAGGT19000.029.1289738
AGCCTCC8550.028.94549444
TCTCGGT37200.028.9094243
GGTTCGT19750.028.70700312
CGGGGCT12500.028.6404272
TCCTCAA26400.028.46406412
Produced by FastQC (version 0.11.2)