FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n02_asyn-l1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n02_asyn-l1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78.433734939759036No Hit
AAAATGTTGATAACTGATGAAAGAATCGCTTAGATAAAAGCATGTGTGAAT11.2048192771084338No Hit
AGGGTGCGTTGAATTGCGACCGTTCTTTGATAAAAGCAGATTGGTGTGCAG11.2048192771084338No Hit
GATGACGTGTAATCATCATAATATTAAAATTATTAACGAGTAAACGCCCCG11.2048192771084338No Hit
TATAATAAGGATTTTGTGAACATCTGACAGACAGGCTCGTTCGTGTCTATT11.2048192771084338No Hit
GGTGGTGGTGGTTGAGGTGGGGGTGGTTGGTGGTGGGTGGGGGGTGGGGTT11.2048192771084338No Hit
AGAGATGAAGACCTTCGTTTATTGAAAAAAGGGAGACCTACTAGGAAGACG11.2048192771084338No Hit
GGAGGTCCCGGGTTCGAGTCCCGGCTCGCCCCACATTGTTTTTACAATGAG11.2048192771084338No Hit
GGGTTAGATGATGATGAAGCAGAGGAGCAAGCCATGTACACCCGAAAATTT11.2048192771084338No Hit
TACTGTATCCGAATATCAACTTCTTCATAGTTACTCAACTTTCTCGCATCT11.2048192771084338No Hit
ACAAGTATTTACTCCATAACAAATTTTGAATAAATATAAATATTTTAAATA11.2048192771084338No Hit
TGGAAATGTCGGAAACAGAACAAAAGATTCTAGCTACAAACCCTATCATGG11.2048192771084338No Hit
AGATTTGGTAAGAAGTGTTAAAAGAAATTAATATAGTATAAAAAAAATGAA11.2048192771084338No Hit
AGACAAAGAGACTAAACTTGGCAAAGAGTTAATCTAAATCTTGTCAAACTA11.2048192771084338No Hit
GGTAAACATGAGTGCTTGTATAAGTTTAGAGAATTGAGAAAAGCTCATTTC11.2048192771084338No Hit
ATGGATCAACATTTCCATGATAGGAAAGCCTCATCATACTAAAGCACTTTT11.2048192771084338No Hit
TGGTTATATAAAAAATAATAAGAAAAGTAAGGCAAGGAATAGAATAGAATA11.2048192771084338No Hit
GCGGCGCTACCATGAGAAATGGGTGAATGTTGAGATAATTGTTGGGATTCC11.2048192771084338No Hit
CGCGGTGAGACCGATGAAACTATCCTGTTGCACTACCAATTGAACGACACG11.2048192771084338No Hit
CGGCGTCATCGGTACCGGTTTGCTGGTGGGGACTTCATCCACGCTTCATAC11.2048192771084338No Hit
ACAGATCTTTGCTGTCAGATGGCACCAATGGGCAGTTTGTGCTGGAATCTA11.2048192771084338No Hit
TTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGTTTGGTTCGCTTTGAGT11.2048192771084338No Hit
AGCCAACCTCACCAAGTTTCAACCACAGTGCTTTTCATGTTATGGAGGAAA11.2048192771084338No Hit
AAATTCAATCACATCATAAGTGTAATCATACGAATATACATCATTGACAGA11.2048192771084338No Hit
GAGGGAAGTTTTAAGGGAAGTAGTATGGGAAATCGAGCGTACAGTAGTAAA11.2048192771084338No Hit
TTCTTTTCTTGTTCAAGACGTAATGAGTGGGTATAAGAGGGGCTATTTAAA11.2048192771084338No Hit
CCTTAACCAAAGACAAGTCGGGTGGCTCCTGTCCGTCCGACTCATGCCCGC11.2048192771084338No Hit
CCTAAAGTACATCATTATCCTATTCCTAACTCCTCATCTAAAACTTTATCC11.2048192771084338No Hit
ACCCAAATTATCAGAATTTAGGCCTTCTACTTTATAGTGTCCTTAGACCTT11.2048192771084338No Hit
GTGGGAAAGTATTCAAAATAATGTAGAATACTAGCTTAAAGATCTAACGGT11.2048192771084338No Hit
AGCTGGATTAATTGATGGTGATGGATATTTTGGTATTGTAAGTAAGAAATA11.2048192771084338No Hit
CCATAAAAAAAAAAACTAACAAGAAAATAGTTGTATAATATTTCTTTAATT11.2048192771084338No Hit
GTGAAATTACAACTACATTTGTCACCAAAAACATTCCAACCACTTACCTAA11.2048192771084338No Hit
ACGATAAATAACTACCATAGTATCCAATAACCAAATTGTAAACTATCTTAA11.2048192771084338No Hit
CGTTGCTGAGTTGCGACCTAATGCACGTGAAGGTACTGCAAGTCCTGGAGC11.2048192771084338No Hit
AGTATTATTAGATTACAAAATTACTTCAGCAAGAATACCAAGAATAATTAT11.2048192771084338No Hit
GGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.2048192771084338No Hit
ACGTGTTACCAAAGATTCGATATTTTTGTAAGTAGTATTTCAGGTGTATGC11.2048192771084338No Hit
CGGGTATGGACGGAATATGGTTCGCATCGAACCGTTCACTCAACTATTAGT11.2048192771084338No Hit
AGGTGTGCGATGTATGTAGGTTTTCAAATGTCTTTAATATTACAAAATAAT11.2048192771084338No Hit
GGGAAAAATCAACCTCTCTAGGGCTAAGCCACCTTTAAGTACCAGTGTGTA11.2048192771084338No Hit
GCGCATCGCTGTCAGAGTATAATACATTTTTAACACCCAGTACTTGAGTCT11.2048192771084338No Hit
AAGAAAAAAAAAAAGCAAAACGCACGGTAATAAAAACGAAAAAAATAAAAT11.2048192771084338No Hit
TGAGGAAAAGTAGTTGGGAGGTACTTCATGCGAAAGCAGTTGAAGACAAGT11.2048192771084338No Hit
TTGAGGGACCATTACTAAATAAAAAGCAACTAGAAGAATTAGTTAAATCAA11.2048192771084338No Hit
GGCAAACGAATCGTATAGTTAAATCTTTTTTCAAAATACATAAATGGTAAT11.2048192771084338No Hit
AGCTTGAAATTCAAAAAAATTACATGGGCTCATAATCATTTGATAGAACGA11.2048192771084338No Hit
TAGAATCATCCATACCACCCAAACTGGAATTAGTCTGACGAGAGTGTATGT11.2048192771084338No Hit
AGCGAGAGCCCTTCTTGCATGATTATTCATATATTTCAATCCGAGCAAATA11.2048192771084338No Hit
GTGCAGTATATACATGTATACTTAAAAGATATGGAATGGTTGGCGAAGTAA11.2048192771084338No Hit
GAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCTACTA11.2048192771084338No Hit
CCCCAGCATCGGCACTCGATCCTGCCAACTTTATACCATTCAATTGACCCA11.2048192771084338No Hit
GGATTCAGTGGCCCAATTGATGAACAATTGGGATTCTATAATAAACAATAT11.2048192771084338No Hit
GGGTGTGGAGGGGGGCGTGGTTGGTTTTGGGAGGGGGTGGGGTTTGGGGGG11.2048192771084338No Hit
CCTCTGCATGCCACCTACCGACCAACTTTCATGTTCTGTTTCGCCCTACCT11.2048192771084338No Hit
AGGTGCAAATTGAAAAGGATGCCGTCCTTTTTCCGTCTTCCTTCTTTTTCG11.2048192771084338No Hit
ATTTCATTTTATTAAGAAAATTCCAATAAACAAGTTAAAATTATTTTAACA11.2048192771084338No Hit
ACGTGTAGATCAACAAGCCAAGTGTGAACCTTATAAAGGTACTCTGAACTT11.2048192771084338No Hit
ACAGTTCAGCAGATCCAAGAGGATTTTCCACGAGGTAGTGATGGTGAAGAG11.2048192771084338No Hit
TGATACAAAAAAAAACAAAAACAAAAAAAATAAAATATAAAAACAATATAA11.2048192771084338No Hit
GACAGAATTAGAGGTCTTCAGAAAAAAATGTTAGCCTACGAGAGGTTTTTG11.2048192771084338No Hit
CCACCTTACTTCTGCATAGTTATATGATCGTAGCCGACAGCATATTGCGGG11.2048192771084338No Hit
GATGGAAACTGAAACAACACGTCCCAAAAAGCAGCAACAGCAACATACAGC11.2048192771084338No Hit
AGGTGGGTCGAAACAGAACATGAAAGTTGGTCGGTAGGTGGCATGCAGAGG11.2048192771084338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGTGG11.2048192771084338No Hit
ATGAAATGAAGACGTGATACGAATTTTGTGCTTAAACTGAGGTTTGATTTA11.2048192771084338No Hit
NNCACCGGTTAACATTCGTACAATTTTATGAAATGACAACAATGACAGCCA11.2048192771084338No Hit
GAGGGCAACGTCGACAACGCTAAAAGAGAAAGGGAGAAACGCGCAAGTGTT11.2048192771084338No Hit
AATTGACTCAACTACAAACGATAGATATAAAAAAATATTCTAATTCACTTC11.2048192771084338No Hit
GCTGAATCTTCCACAATTGATGAAAGTAGCTAGGAAGAGGAATTGGTATAA11.2048192771084338No Hit
ACGTGAATTGATTTCGTAATTGTTCTGTTCAGATATATTGAATTATGTGAG11.2048192771084338No Hit
CGTGGTACAAAAAACCGAAAAGCTACTATTGTCATTACAGCATCATCTACA11.2048192771084338No Hit
GCGGTACCACTCACGAAAGGTAGGAGGACCGAAGGCGTGTTAGTATGTTTT11.2048192771084338No Hit
CGATGTAATTGTCGTTCCAATAAGTTGCTCAACTTAGTAAAACTATGATCA11.2048192771084338No Hit
GGTAGGGCATTTTGACAGTTTAACGTTGGTCATTTGATGAACTGTCTTGTT11.2048192771084338No Hit
TTTTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGA11.2048192771084338No Hit
TGGGGTTTCCTATACCCTCGAGGGGAACTTTTAGGATATTCTGTGTATCTC11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT50.045.025
GGATGCC50.045.017
TCCTTCT50.045.039
GTGCAAA50.045.03
AAATTGA50.045.07
TTCCGTC50.045.031
TGCCGTC50.045.020
TTTCCGT50.045.030
AGGATGC50.045.016
TGCAAAT50.045.04
GAAAAGG50.045.012
GATGCCG50.045.018
TTTTCCG50.045.029
TTTTTCG50.045.045
TTTTTCC50.045.028
CCGTCTT50.045.033
CCGTCCT50.045.022
TTCCTTC50.045.038
AGGTGCA50.045.01
GTCCTTT50.045.024
Produced by FastQC (version 0.11.2)