FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n02_12-2_45m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n02_12-2_45m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5547327
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184470.33253853612739975No Hit
GCGGCGCTACCATGAGAAATGGGTGAATGTTGAGATAATTGTTGGGATTCC69420.12514135186189673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGC14750.038.189741
GCGGCGC17900.036.2525141
CGGGGCT17950.029.9791012
CGGCGTT12650.029.3894941
GGGGCTA20500.027.8843983
GGGCTAC21000.025.501444
CGGCGCT25550.025.2034662
GCGTTAC12350.024.9652023
CGGGGAC6650.024.7343161
CTACCAT63100.024.1029157
GGGGCGC6600.023.8975161
GCGGAGC7500.023.733651
CTCGGTG15000.023.5485744
CGCTACC33900.023.3622575
GCGCTAC34050.023.1951454
GCTACCA64400.023.0923356
CCATGAG62000.023.04212410
GCGGGAC5950.022.7212891
CGGCGTG3700.022.5319461
GGCGCTA35450.022.4098993
Produced by FastQC (version 0.11.2)