FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n02_12-2_40m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n02_12-2_40m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14545346
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1012420.6960439442279338No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG423620.29124092338539076Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC60850.036.27103844
GTCGCCG61600.035.61028745
GTGGTCG72250.030.51685742
TGGTCGC75550.029.0647743
CTCGGTG80450.026.84623138
CCTCAAC48450.026.1433813
TCTCGGT87200.024.97622937
ATCTCGG88900.024.37224436
GGTGGTC92200.023.84024441
TCGGTGG94800.023.35212339
CTCCTCA54200.023.28700411
CGGTGGT96850.022.90468640
AGATCTC137850.021.78948434
GATCTCG100100.021.60039935
CCTCCTC59600.021.13943310
AGACTCC31700.020.36921144
ACAACTC56350.020.24305318
TAGATCT161400.020.04579733
AATCTAG60250.019.82853129
ACCTCCT52200.019.7832099
Produced by FastQC (version 0.11.2)