FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n02_12-2_35m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n02_12-2_35m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9782260
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259770.2655521321248873No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG131910.13484613984907373Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCAAC53750.033.69492713
GGTCGCC24900.031.71476644
GTCGCCG25250.031.27515845
CTCCTCA58750.030.48308811
CCTCCTC59750.029.89806210
AACCTCC48200.028.1937278
ACCTCCT49350.028.1750019
CACCCCT15500.028.1612976
TAGACTC36600.027.29327443
AATCTAG65000.027.10177429
CTCTCCA42300.027.059881
AGACTCC36150.027.0106644
CCACCCC15800.026.915255
CGGGGAG13500.026.7047621
ACAACTC59200.026.52682518
CCAAACC51050.026.4453435
TCCACCC15900.026.3233184
AGCCTCC20700.026.19391844
TCCTCAA70300.026.0187712
GCCTCCT20100.025.9684345
Produced by FastQC (version 0.11.2)