FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n02_12-2_30m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n02_12-2_30m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649898
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG439240.29982461311334724Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG357580.2440836106845249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCAAC143500.037.5645713
CTCCTCA145750.036.44491211
CCTCCTC147700.035.96399310
GGTCGCC78450.033.8975344
GTCGCCG78200.033.89081645
ACAACTC137150.033.7928918
ACCTCCT122000.033.6738939
TCCTCAA160500.033.656212
CCAAACC118550.033.4033625
CTCTCCA95850.033.289971
AACCTCC120400.033.1684958
AATCTAG154000.033.14867829
AGCCTCC50900.032.7966644
GCCTCCT50150.032.6590845
CCACCCC36400.032.080795
CACCCCT37250.032.0126276
AGACTCC80950.031.96130844
CAACTCA129500.031.86243819
TAGACTC82400.031.8084343
AAACCTC127050.031.5570077
Produced by FastQC (version 0.11.2)