FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n02_12-1_45m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n02_12-1_45m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6998295
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG838821.1986062319464954No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG252600.360945058760741Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC34150.038.40875644
GTCGCCG34300.038.30637745
GTGGTCG38700.033.95113442
TGGTCGC40200.032.57236543
CTCGGTG42000.030.74698438
TCTCGGT46850.027.85332137
ATCTCGG47200.027.78978336
GGTGGTC48500.027.13671141
TCGGTGG49350.026.85166439
CGGTGGT50250.026.72910740
GATCTCG50900.025.37224435
AGATCTC57550.022.79229434
GCACACG8450.022.36579118
AGTGTAG66200.020.05119129
TAGATCT66150.019.93087433
GTAGATC67800.019.41264732
AGGGGTA13050.019.339131
AGGGGTG14050.018.9249671
GTGTAGA72450.018.53868730
GAGTGTA74200.018.13216428
Produced by FastQC (version 0.11.2)