FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n02_12-1_40m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n02_12-1_40m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9767592
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1165741.1934773688335878No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG749410.7672413016432299Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG93950.040.6142645
GGTCGCC95600.040.0544844
GTGGTCG102450.037.4865542
TGGTCGC102500.037.31442643
CTCGGTG106450.035.54883238
TCTCGGT112400.033.6682337
GGTGGTC114900.033.38535741
CGGTGGT116150.033.1033840
TCGGTGG116550.032.93168639
ATCTCGG117600.032.2558636
GCACACG18550.029.10904318
GATCTCG137700.027.5312935
AGAGCAC41400.025.27046215
AGATCTC151700.025.18343434
AGCACAC27400.023.89502117
GAGCACA43300.023.53819316
TAGATCT176550.021.86816633
AGTGTAG177450.021.71910529
GTGTAGA182900.021.20680430
CACACGA15750.020.9989419
Produced by FastQC (version 0.11.2)