FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n02_12-1_20m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n02_12-1_20m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7133183
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1087461.5245087641800301Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG769121.0782283308867864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG127350.041.51672445
GGTCGCC131300.040.38768844
GTGGTCG136400.038.94357342
TGGTCGC137500.038.6156643
CGGTGGT147950.036.08661340
GGTGGTC149050.035.6079641
CTCGGTG152500.034.46356238
GCACACG21700.034.0065218
TCGGTGG159100.033.55712539
TCTCGGT159650.032.879237
AGAGCAC48750.032.58249315
CACACGA17500.032.1398619
ATCTCGG164000.032.07547436
AGCACAC28700.031.27822717
GAGCACA49650.031.17621216
CGGCGCT13300.030.156271
AAGAGCA79800.028.41919714
GATCTCG185300.028.30344435
ACACGAG20350.027.74944720
AGATCTC202600.026.18664434
Produced by FastQC (version 0.11.2)