FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n01_asyn-s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n01_asyn-s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4882630
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77270.15825487493420554No Hit
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG74730.15305276049997646Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAG20700.028.80249227
GAGCGGT38350.028.15783710
AGAGCGG38800.027.7732819
CCGAGAG14400.027.4982329
GCGGTTC39950.026.91748612
ATGCCGA23950.026.4915326
AGCGGTT41550.026.0975311
AATGCCG26850.026.06030825
AAGAGCG41500.026.0210888
CAGCAGG41700.025.46441818
CGGTTCA43050.025.13595213
GCCGAGA23650.025.0195328
GGAATGC33700.024.9692323
CGAGAGA16950.024.95414430
AGCAGGA42750.024.52398119
GGTTCAG44950.024.0737214
GCAGGAA44200.023.51682720
GTTCAGC46600.023.12477115
GAATGCC34550.023.05253224
AGGAATG38700.022.96387322
Produced by FastQC (version 0.11.2)