FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n01_asyn-s1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n01_asyn-s1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8944533
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC20600.019.66187939
CCCGTCC21050.019.34845240
GAGCGGT21950.019.16635110
TGCCGAG13900.018.93782627
TCACCCG22000.018.71730437
CCGTCCA22150.018.2850741
AGAGCGG22800.018.2545729
GCGGTTC24150.017.51350412
CACCCGT24300.016.85310738
GTCACCC25050.016.618136
AAGAGCG27850.015.0252788
AGCGGTT29300.014.66554111
GTCCATC25550.014.61850443
CGGTTCA29900.014.52189613
CCGAGAG12750.014.29358429
AATGCCG21400.014.19339725
CGTCCAT28050.014.03785142
TCCAGTC30350.013.936832
GCCGAGA18950.013.89099228
CAGTCAC30550.013.77291534
Produced by FastQC (version 0.11.2)