FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n01_asyn-l2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n01_asyn-l2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4049637
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG120050.2964463234605966Illumina Paired End PCR Primer 2 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67590.16690384841900643No Hit
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCACACGTC57020.14080274355454575Illumina Paired End PCR Primer 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC43290.10689847015917724TruSeq Adapter, Index 22 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAG30550.031.37361327
GAGCGGT61700.030.37343210
AGAGCGG62450.029.936619
GCGGTTC62600.029.86487412
AATGCCG40500.029.8318425
ATGCCGA35050.029.59159326
CCGAGAG21450.029.36916229
AAGAGCG64050.029.259398
GCCGAGA32700.028.96644228
CGGTTCA64450.028.93780713
AGCGGTT64700.028.93030711
GGAATGC51100.028.79503823
CAGCAGG64050.028.66190518
AGCAGGA63950.028.42633819
GGTTCAG66050.028.3049414
AGGAATG56950.027.18004622
GAATGCC50600.027.16761624
CGTACGA40750.027.05753741
GTTCAGC69800.026.97764615
GCAGGAA66550.026.91042120
Produced by FastQC (version 0.11.2)