FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n01_asyn-l1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n01_asyn-l1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length51
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGACGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTAT22.4096385542168677TruSeq Adapter, Index 18 (96% over 33bp)
AAACTATATTGTATACATAAAGTAAAATAAAACTTATAAACACAATAAACA11.2048192771084338No Hit
GCAAGCACAACAAAAGACGAACATGGACCGGAAAGTTTGTTGAAGATCCGG11.2048192771084338No Hit
CACAAGTATCAGAACAATGAAATGATCGTGATCATTGTGAACAATTAGAAC11.2048192771084338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGTTGTGCCCCCCGGGTTT11.2048192771084338No Hit
CATTAACGTAAGCAATTGAATAGGGTCAGTAGTTCTAGCTGTTTACTCTTA11.2048192771084338No Hit
AAAAATTAAGAACGATCTCAATATCGTACAGAAAGACCCACCTATTATGTA11.2048192771084338No Hit
GGTAATCATGTCATATTGTTGGGTGATGAATATAAGTTGTAGATAATAACA11.2048192771084338No Hit
TTCACTTGTCTCTTACATCTTTCTTGGTAAAATCGTAGTTCGTAGTATTTT11.2048192771084338No Hit
CATCTACTAACTAGTATTTACATTACTAGTATATTATCATATACGGTGTTA11.2048192771084338No Hit
ATTCGTTTTTCTTAGAATCTTTCCACGGTCCTATCTCATAATCACCCTCTG11.2048192771084338No Hit
CTAGTCAAGCTCACCATGCAGCTCAACATCATCAACAACAACCTACTAAAC11.2048192771084338No Hit
AAAACTCTACGTCCTCGGTGGACACCGTTAAAGACTGTTCGGAATCCGCGA11.2048192771084338No Hit
GAGGTAGTTTCACCGGCAGAGTTTGTACCTGTAGAGCAAACCGTAGTAGTA11.2048192771084338No Hit
AAGATGTTAAAATGAAAATAAATATACAAAAGTTTAAATTTAAATATAAAT11.2048192771084338No Hit
AGATACTACTTCACCTAGACACTCGTCTACCTTCACTGCACGAAACCAAAA11.2048192771084338No Hit
GGTCAACCTTACTCCCAGTGAGTTTGGTGATATGGGATTATACGAAAATGC11.2048192771084338No Hit
TAATAAATAAACTAAAAACAAGGAATATAATACTCGGTTGAACTACTTAAT11.2048192771084338No Hit
CGCGCTATGTCAGTCTGTACCTTTTACCACAATTGACAAGCCTTCCCGCGG11.2048192771084338No Hit
CGGAAAGATAAATGCCGTACCATTTCTGCCAGTAGCGACACCACACATTGT11.2048192771084338No Hit
AAAAAATAATTAAAATTATATATTATTTTTATAAATAATATATATAATATT11.2048192771084338No Hit
TGGATAAATAAAATCAATACCTAATAAAATACAAACTTTATTAAATTGTAC11.2048192771084338No Hit
CACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGT11.2048192771084338No Hit
AACCTAGTCAACACACATAACTGAAGTCAATTTGACCAGGTGCAACTGAAA11.2048192771084338No Hit
GGTTTTTGTGTTTTTGTGTTGGGGTGGTTTTGGTGATTTTGTGGTGTTTGT11.2048192771084338No Hit
CACAAATAAATACAAAATATCCGCGCCTGGACCGCACAAACAATGTGCAAT11.2048192771084338No Hit
ATCCAGGAACCAAATTCTAGAAAAAGATACAATCCTGCAAAAGCGATCGCA11.2048192771084338No Hit
CCTTTGAGAGCGCAAGGAGTACAGCCCATCTATGCTTTTGGGGTGGGCGGT11.2048192771084338No Hit
CGATCTTACGAAAACACGGCTGAACAAACTGAAATTCTACCAAGAATATTA11.2048192771084338No Hit
GCAAAATTATAACGAAATAAGTACAAACGTAACGCACCTAATTACTCAAGT11.2048192771084338No Hit
CCCTAGAAGCTGAATTGCTTGCCCTTTCGTTTACTTTGGCCTCAAAACTTG11.2048192771084338No Hit
CAAAGTTGTAGGCATCAATTCCCAGCAATTCGCACGCCAGTTTCAGGTTTG11.2048192771084338No Hit
ACTTGCATTATAGAGAAGCAGATCTTGCAAATTAGTCCCATCTGGTTCATA11.2048192771084338No Hit
AAAATACCAGATAAAAGTTCTGTCAACTGCGAAGTTCACTATAACCCCTTA11.2048192771084338No Hit
GCTATATACCAAGAGAAGTTACTTACGTTCCCAAGTTATACGGACGTTAAA11.2048192771084338No Hit
CGGTTTATTGTAATATTTTGTGTGATTTTGGGTGGGGGGGGGTGGGGGGGG11.2048192771084338No Hit
CGCATTTATTTGAGTCATTCTCTGCCGCTTTATTATTAAAAGTCTGTGTGC11.2048192771084338No Hit
CGGACCTTAATTTTTCGTACTTTTCAAAAAACACTGAAAAACTGCGAAAAA11.2048192771084338No Hit
TGTATAATCAAAGTTAGGACTCCAATTGAGGGGCCATCTTTCCAGCACAAA11.2048192771084338No Hit
CTGCCTACTCGTCAGGGCATCATATCAACCCTGACGGTAGAGTATAGGTAA11.2048192771084338No Hit
CTCCTGGTAATGTCTATATGGCCGCTATGGTCCTGCTATCATGCTATTGTT11.2048192771084338No Hit
AGATAAGGGAAATAGCCACAGTAAAAAAATGCTTAAAGCACTTCTAAGTGA11.2048192771084338No Hit
TCTTATTCCTTCCCGCTTTCCTGCACTAACATTTTGCCGCATTACACTATA11.2048192771084338No Hit
GCAAAAAGCACACCATTTCGCCCAGCGCGCACATGATCGGGTAGATCACCG11.2048192771084338No Hit
TTCGACTGGAAAATTTTTGCGAAGCACTTAATCGGAGAACTTTGGTGGCTT11.2048192771084338No Hit
TTCTATACAACTAAACTTAGGCGCATCAACAGTTGAGTAAGTTGATTGTAT11.2048192771084338No Hit
CAACCATAATTTGACGTTCGTACTCAGGATCGGAACTCTTACCGATGCAGA11.2048192771084338No Hit
CGTGCAAACGCTCTCTCTCTCTCTCAAGCTATATAAGTGGCACTCGTCTTA11.2048192771084338No Hit
TGGTGCTTCGTCTTTATTATTCTCTTCCTTTAAGGCTAATGCATGAGCCAA11.2048192771084338No Hit
ACAAGCAAACTGGTTGATGTTCTTCCAGTACGGTAACGTCGAGGTAAACTA11.2048192771084338No Hit
CAAAATTATTAAATTTCTTTAATGATCCTTCCGCAGGTTCACCTACGGAAA11.2048192771084338No Hit
CGCCGCACCAAATATTAGCCTGGAAAGGTTTATCTGCATATGGTTATCAAC11.2048192771084338No Hit
CATCGGATTGTTGGATCTCATTTGACGCAATGAACGATTGATATGAAGCAG11.2048192771084338No Hit
ATATAACTAGAAAATAAAAAATAAAATAACAGACTTATGAAATTGTAATAC11.2048192771084338No Hit
GCTAGTAATCCACCAAATCCTTCGCTGCTCACCAATGGAATCGCAAGATGC11.2048192771084338No Hit
CCCGCGACAATACATTCATATAAAGCTTGGTCTGCTACGGCGACTACGCTG11.2048192771084338No Hit
AAGCAAAATAATATTCCCTTTATCATAATATCATCCATGCAAATACCCAGC11.2048192771084338No Hit
AATACAAGAAATAAAGTAATTTTTAATAGTTTTTGTATAATAATAATAATT11.2048192771084338No Hit
TAAAATTTCATACTATAACAACTCCGACTAAAACCCAAGCGGTACACATGG11.2048192771084338No Hit
ACCTTAACACTTTGCAATGGATCACAGTACGACACAATTCAGCCCGGCACA11.2048192771084338No Hit
GAATGCACGTGGTTCACCTCATGTGCCAGACCAGCAGCACCATTATGCAGT11.2048192771084338No Hit
CGAAATTTATATATATATATATATGTATCACTAAGCTCCTAATTAGACTGC11.2048192771084338No Hit
CAATAAAAGCATACCTATTGTCAAACCTTTGAACAAATCTACAGATCTGAA11.2048192771084338No Hit
GGAGGCGGCTTTTTGACCGCCTCCAAACAATTTAGACTAGGCGCCCACAGC11.2048192771084338No Hit
ATTCTCTACGTAAATTATCTGGTGTGATGTGTTCTGCATCACCTTCAGTAG11.2048192771084338No Hit
ACTTGCGTAAGCCATAGAACGGAAATATCATGTTCCCCTTGCCGCATGCAT11.2048192771084338No Hit
GAATAAAAATCAGATGCTCCTTAGAATGACAAATCAGTGAGTACATAACAA11.2048192771084338No Hit
AAATTTCTTTCTTTTCCACAATGGAAGAAAAGTAGTTTACAATAAGGGCAG11.2048192771084338No Hit
ATAAGAAATTAAGGGTAACTGGTTAGTACTTTTGCGGGGAATTACATCCTA11.2048192771084338No Hit
TAAAATGAAACAAAATAAAAATAAAATATCAAGCAAAATTATAAAATATTC11.2048192771084338No Hit
AGCTATTTCCAAGGTGAAAAAAGCTGACATGGTCGTTTATTCTAACGGTGC11.2048192771084338No Hit
AACTTTCCAATAGAACCACCACGTTCTGATGAGAAAGCAAGCATCCAAAAT11.2048192771084338No Hit
CTATCGCATCGATTGAAGATCTCAGATATAAATCTACAGCAGACTAATATG11.2048192771084338No Hit
AACCAAACTAAGGCTTTGAGGTAGAGTTTACTTGACAATAGACCAACTCAT11.2048192771084338No Hit
GCCGCCATCATACATTATTCGCCGCCCGTAGAGAACTCTTCGATGATTGAT11.2048192771084338No Hit
CCGACATTCTGTCCCACATACTAAATCTCTTCCCGTCATTATCGCCCGCAT11.2048192771084338No Hit
GTCCTGGTGTTTATGATAAAGGACATATGACATGCGTATTAAGTCACTTCC11.2048192771084338No Hit
TAAAAATACTCTAGCTATTATTTACAAAATTTTTTTCAGTTTTTGTTAATT11.2048192771084338No Hit
GAAAAAACAAATTTACACATGAGTATTGATCATATGTACAACAATAATCAA11.2048192771084338No Hit
CACTCTTCTATTGCATTTGGTTCGAATACGCTCTCAATATTAAATCAAAAC11.2048192771084338No Hit
TTTTGATTATAACGTTGATCTACAGTGTAATGAGCACGAAAATTTTGACTT11.2048192771084338No Hit
GAAGGGTCTCAATAATTTGGGTCTGTAGAGTGAAAGGAAGAGACCGTACCG11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC50.045.034
ACAGCAG50.045.036
ACTAATA50.045.043
AATCTAC50.045.031
TCGATTG50.045.09
TAAATCT50.045.029
ATCTCAG50.045.019
CAGCAGA50.045.037
AAATCTA50.045.030
TACAGCA50.045.035
GATATAA50.045.025
ATCTACA50.045.032
TATAAAT50.045.027
TATCGCA50.045.02
GAAGATC50.045.015
TCTACAG50.045.033
GATTGAA50.045.011
AGCAGAC50.045.038
ATAAATC50.045.028
AAGATCT50.045.016
Produced by FastQC (version 0.11.2)