FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n01_12-2_45m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n01_12-2_45m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5547327
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT15800.023.4937410
AGAGCGG16050.022.9876259
GCGGTTC17200.021.58165412
AGTCACG16500.021.1379635
CACGTGA16700.020.75063138
GTCACGT18400.019.32263436
AAGAGCG18800.019.266258
TCACGTG18750.018.7219237
TGCCGAG11350.018.6332527
AGCGGTT20500.018.4367611
CCGAGAG9250.017.99908829
CGGTTCA21400.017.55625513
AATGCCG14650.017.20080425
CAGCAGG21850.016.98860718
ACGTGAA20750.016.91739839
AGCAGGA22100.016.89898719
GGAATGC18400.016.7521723
ATGCCGA13350.016.68469826
ACGTCTG21500.016.53419522
CGAGAGA10850.016.17414930
Produced by FastQC (version 0.11.2)