FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n01_12-2_35m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n01_12-2_35m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9782260
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG325140.3323771807332866Illumina Paired End PCR Primer 2 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC134480.13747334460543884TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116920.11952248253471079No Hit
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCACACGTC114110.11664993569993028Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT125850.031.06922310
CCGAGAG53550.030.9229629
TGCCGAG73200.030.8898327
ATGCCGA76150.030.6981926
GCGGTTC128650.030.27060912
AGAGCGG130100.030.0888659
AATGCCG86950.029.6540825
AGCGGTT131950.029.5306111
GGAATGC109150.029.18860623
CGGTTCA133850.029.1786513
CGAGAGA59900.029.10909730
AAGAGCG135500.029.0725388
GCCGAGA76250.028.7985428
CAGCAGG135050.028.70311418
GGTTCAG137450.028.39820314
AGCAGGA135150.028.3496719
GAATGCC106250.027.71916424
AGGAATG121800.027.67129522
CAGGAAT134350.027.31374521
GCAGGAA141100.026.78842720
Produced by FastQC (version 0.11.2)