FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n01_12-2_30m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n01_12-2_30m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649898
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG914260.6240726044645498Illumina Paired End PCR Primer 2 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC458960.31328545768714566TruSeq Adapter, Index 13 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG372900.2545410213777598No Hit
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCACACGTC304150.2076123669939545Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAG143350.032.5987129
TGCCGAG193450.032.1580327
ATGCCGA203050.031.92340926
GCCGAGA195350.031.48822628
AATGCCG233150.031.32435425
CGAGAGA159050.030.72481230
GAGCGGT370500.030.53127510
AGAGCGG375300.030.1887459
GGAATGC298300.030.10969223
GCGGTTC375400.030.04885712
CGGTTCA377550.029.85390513
CAGCAGG370800.029.82106618
AGCGGTT381100.029.65845711
AAGAGCG383650.029.5553578
GGTTCAG383300.029.41779514
AGCAGGA375200.029.3342419
AGGAATG325050.029.18921722
GAATGCC288100.028.88750624
GTTCAGC389650.028.8113615
CTCGTAT77700.028.66862943
Produced by FastQC (version 0.11.2)