FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n01_12-1_50m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n01_12-1_50m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4389
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAGATCGGAAGAGCACACG170.3873319662793347Illumina Paired End PCR Primer 2 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.2961950330371383No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCGGAAGAGCACACGT60.1367053998632946TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCAAATCTCGTATG60.1367053998632946TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCAAATCTCGTATG50.11392116655274549TruSeq Adapter, Index 15 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)