FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n01_12-1_45m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n01_12-1_45m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6998295
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC249480.3564868300064516TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG35650.034.02109540
CGTATGC40400.031.46698645
CTCGTAT41150.030.67540743
ATCTCGT43450.028.43080941
TCGTATG44650.028.1192644
TCTCGTA46750.026.13514342
GGCTACA49600.025.58591735
CGGCTAC50600.025.16866534
GCTACAT50750.024.47444236
ACGGCTA52900.024.28548233
CACGGCT53000.024.1972132
GTCACGG53350.023.82743330
TCACGGC53750.023.81754731
AGTCACG54500.023.53123329
ACATCTC53350.023.0291239
CAGTCAC64300.020.11949228
CTACATC62350.019.99323537
TACATCT66300.018.76827838
CCAGTCA69250.018.6814827
TCCAGTC69300.018.63540326
Produced by FastQC (version 0.11.2)