FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n01_12-1_40m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n01_12-1_40m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9767592
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC716070.7331080167967704TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC97050.037.0712545
TATCTCG96450.036.51225740
CTCGTAT99600.034.92609443
ATCTCGT100250.034.90360641
TCGTATG100800.034.77746644
TCTCGTA106600.032.8876142
GCTTATC113650.031.99571237
AGCTTAT129750.028.5976936
TTATCTC126600.028.31448239
CTTATCT129050.027.58498838
TAGCTTA142150.026.13351635
CACTAGC158550.023.61301232
ACTAGCT158550.023.570833
CTAGCTT158250.023.55933834
TCACTAG164650.022.77906631
AGTCACT169250.022.25358429
GTCACTA171400.021.92159530
CGTGTGC24550.021.7321931
CAGTCAC176500.021.45432328
CCAGTCA198300.019.1411427
Produced by FastQC (version 0.11.2)