FastQCFastQC Report
Tue 31 Jan 2017
HH2KGAFXX_n01_12-1_20m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2KGAFXX_n01_12-1_20m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7133183
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1030171.4441939874527263TruSeq Adapter, Index 7 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98020.13741411092355263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC125350.040.58279845
CATCTCG125700.038.5415640
TCGTATG131900.037.69782344
ATCTCGT129700.037.33505241
CTCGTAT131900.036.9653743
TCTCGTA134150.036.1296242
GATCATC156500.032.06328637
TCATCTC160950.030.33816539
AGATCAT180150.028.6036736
CAGATCA189150.027.45526735
CGTGTGC32850.027.412731
ATCATCT204750.024.02391438
TCACCAG223550.023.3990831
AGTCACC226600.023.09438529
CACCAGA227750.022.97761232
CCAGATC228300.022.82498734
ACCAGAT230550.022.7086333
GTCACCA233700.022.36372230
CAGTCAC236050.022.3510828
GTGTGCT40400.022.3386362
Produced by FastQC (version 0.11.2)