FastQCFastQC Report
Thu 29 Jul 2021
HH2K3DRXY_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2K3DRXY_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069548398
Sequences flagged as poor quality0
Sequence length10
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATACAGG53144659225.679350746935274No Hit
TTTATCCTTG47896308023.14336212010636No Hit
CACAACATTC44898967221.695055425323762No Hit
GCGAGAACGT44642982821.57136447890889No Hit
AGATCTCGGT618156592.9869153608457917Illumina Single End PCR Primer 1 (100% over 10bp)
AAATCGTTGG131522080.6355110135481837No Hit
AGACCATCGG128341100.6201406071200274No Hit
TGTGACGATC127350740.615355215287891No Hit
AACGAACTGT108245620.5230398095768524No Hit
GGGGGGGGGG67130090.3243707180990507No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)