FastQCFastQC Report
Thu 29 Jul 2021
HH2K3DRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2K3DRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069548398
Sequences flagged as poor quality0
Sequence length10
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCACTTA52659565825.444954972248972No Hit
TGCGCGGTTT47775085823.084787891971782No Hit
ATGGCTTGTG43971444921.24687924307243No Hit
TCCGTTGGAT43794544121.16140127108059No Hit
GGGGGGGGGG739825723.5748171954565713No Hit
GCCGTCGGTA132824230.6418029659434908No Hit
TCGGCTCTAC128459540.6207129058887562No Hit
CACGGTGTAT78292580.3783075577051569No Hit
ACCGTAAGTA76353450.36893773575813715No Hit
CACGGTGAAT50155880.2423518099333669No Hit
CGCGCACTAA40202000.19425494005770044No Hit
ACCGTAACTA32016780.15470418585494708No Hit
TGCGCGGTTA24426420.11802777854147097No Hit
TCCGTGGTTA21216270.10251642348883111No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)