FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11350155
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3725073.281955180347757No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN263060.23176775999975333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC36400.027.6880867
ATACAGG60000.026.9559466
TATACAG76550.024.7335725
GTATATA120050.020.8310891
TGCAGTC16700.019.9719687
GTATTAG33700.019.1988771
GACTAGG11200.016.1847278
TATATAC138100.015.9215393
TAGGTAG22450.015.8332935
GGAACTT61500.015.534006145
ATGCAGT28700.014.9097746
GTATTAT80300.014.4850561
GTATAGA43600.014.1725561
ATATACA138950.014.1439044
CTAATAC44650.013.6519263
GTAGTCA19350.013.4897818
GTCCTAG9357.094059E-1113.2176131
CTACGTA22550.013.1831968
CAGTGTC14300.012.6759259
ATAGAGC26000.012.5527856
Produced by FastQC (version 0.11.5)