FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_s.EP062.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_s.EP062.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3890260
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG65760.16903754504840293No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA38960.10014754797879834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA13200.030.2079779
TAGAGCG14650.027.7129827
ATACAGG66050.026.0140746
TACAGGC40900.024.8163327
TATACAG87400.023.7239785
TGCAGTC16700.022.5745857
GGATACG17400.021.6664129
ACAGGCA47500.020.9102788
TACAGGT32050.020.3585747
GTATTAG40400.020.2818781
GACTAGG13850.019.8914628
CAGGCAA49050.019.8060879
GTATATA141350.018.9809061
GCCGACC4253.279456E-818.764484145
ATAGAGC28100.018.0602866
TATATAC168150.016.5566643
ATTATAC76150.016.4707983
GTATAAT67600.016.3045181
TAGGTAG23750.016.1787575
TCGTACG6306.7484507E-1016.110927
Produced by FastQC (version 0.11.5)