FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_s.EP060.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_s.EP060.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4607292
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG79300.17211845917298058No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA16950.030.7953629
TAGAGCG18700.028.301137
TACAGGC49300.027.9401827
ATACAGG77450.024.8054456
ACAGGCA56100.024.2950368
TATACAG101100.023.5921385
CAGGCAA62550.021.673899
GTATATA169250.019.281811
GTATTAG39200.019.2402731
TATATAC197550.017.6881793
ATAGAGC35250.017.0703146
CTAATAC49850.016.7242163
TGCAGTC21000.016.5708377
GTATAAT83750.016.365941
ATATACA167050.016.3175324
ATTATAC91550.016.0749953
TATACCG28350.015.5990925
GGAACTT85150.015.32535145
TACAGGT41350.015.078067
CTACCCC15750.014.7293154
Produced by FastQC (version 0.11.5)