FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_s.EP050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_s.EP050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13168842
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG409900.3112650299851726No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA242210.1839265745613775No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT188300.14298903426740178No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT168210.1277333268938909No Hit
GTATTAGGTAGTCAGATGAAAGATGAATAGACATAGGAGTAAGAAAACAT159480.12110404240555092No Hit
GATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTC149720.11369260865913647No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC139280.1057648045287505No Hit
ACCTAATACTATGCAAAAATGTAAAATCATCACACAAAACATAAACAATC137010.10404103868813978No Hit
ATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCT132490.10060869437115276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC184750.036.1407247
ATACAGG296300.033.0800936
ACAGGCA210750.032.0260778
TAGGTAG81950.030.1666495
TGCAGTC62650.029.7395047
TATACAG364500.029.6552645
CAGGCAA230250.029.2193159
TACAGGT108950.026.084387
GTAGTCA94400.025.0361358
GAGCGCA42650.024.9873379
GTATTAG151800.024.890451
GCCGACC11050.023.618841145
TAGAGCG46100.022.8028327
TCTCTGA96150.022.092085145
CACCCGC48000.021.900051145
CAGCGTG41350.020.8637859
GTATATA515650.020.5617081
TATATAC622400.020.3843443
ATATACA544350.020.3231664
GGATACG65300.020.0949579
Produced by FastQC (version 0.11.5)