FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_s.EP049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_s.EP049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7491862
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG109380.14599841801677607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC70300.025.2661847
ATACAGG112250.024.4783296
ACAGGCA77900.022.5219928
GCCGACC8100.022.376076145
TATACAG147150.021.7766445
CAGGCAA84550.020.6648549
GTATATA267800.018.8732551
GAGCGCA22100.018.3706559
TGCAGTC31300.018.2983387
GTATTAG69050.016.2776831
TATATAC299550.016.1190573
TAGGTAG44600.016.0927125
TCGTACG13600.015.9923127
TACAGGT56700.015.9829117
ATTATAC153350.015.9796633
ATATACA248000.015.7859594
TAGAGCG25200.014.6723117
CAGCGTG18300.014.6581649
GTATAAT135150.014.3794841
TAATACT195600.014.2698964
Produced by FastQC (version 0.11.5)