FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_s.EP026.2A-2C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_s.EP026.2A-2C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8760606
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA255000.015.4139341
GTATTAG77250.015.3018351
CATATAC132350.014.0224583
TATACAG106750.013.8541655
ACTATGC65000.013.1609348
TATACGG81200.012.8565525
TATATAC266750.012.8004313
TACGGTG67050.012.6504267
GTATAAT163200.012.5753691
CTAATAC83700.012.5588733
TCTATAC82750.012.26501753
CTATGCA64600.012.232419
TATATAG158100.012.1974165
GTATAGA87050.012.1629371
ACGGTGT67650.012.1095698
GTGTATA89250.011.9443771
GTATATG142450.011.8108171
TACTATG86300.011.6767527
GTATAGG46100.011.4835551
TAATACT168200.011.4645984
Produced by FastQC (version 0.11.5)