FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_D2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_D2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2573692
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG42600.16552097142937072No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA27880.1083268705035412No Hit
ACCTAATACTATGCAAAAATGTAAAATCATCACACAAAACATAAACAATC27220.10576246108702984No Hit
GTATTAGGTAGTCAGATGAAAGATGAATAGACATAGGAGTAAGAAAACAT25970.10090562507090982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG16350.030.1521845
TACAGGC24400.029.7124027
TATACAG57750.025.2331625
ATACAGG43850.025.1305266
ACAGGCA29700.024.6542938
GTAGTCA18900.024.5496758
CAGGCAA28950.024.5417279
GCCGACC2502.2827844E-623.198542145
CAGCGTG9450.023.015329
GTATTAG27550.022.375711
TACAGCG12000.021.7494777
TCTATAC26700.021.4503783
ACAGCGT11300.021.1720588
TAGTCAG22550.019.9330029
GACTAGG9550.019.7377458
GTATATA92700.019.4804551
GGATACG15750.019.3328699
CTATACA20750.019.2160324
TCTCTGA20650.018.606579145
CTAGGGT6650.018.5330314
Produced by FastQC (version 0.11.5)