FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_D1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_D1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5749198
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG93970.16344888452267603No Hit
ACCTAATACTATGCAAAAATGTAAAATCATCACACAAAACATAAACAATC65020.11309403502888576No Hit
GTATATATCTATTATAATATACGATGAGGATGATAGTGTGTAAGAGTGTA60320.10491898174319271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG34350.029.336495
TACAGGC53550.029.1071117
ATACAGG94800.024.6245166
GTATTAG63600.024.5159551
TATACAG129150.024.3060055
ACAGGCA67050.023.4628688
CAGGCAA68150.022.9777779
GACTAGG25050.022.2845128
GTAGTCA44400.021.3898858
GAGCGCA18600.020.6577539
TAGTCAG50000.020.4441599
GTATATA195600.019.9842281
TGCAGTC24750.019.6254567
GGATACG32700.019.0664949
TAGAGCG20250.018.9745277
AGACTAG29800.018.4891727
TATATAC222250.018.3328423
TCTATAC57450.018.2984033
GTATAGA64600.018.1865351
CAGCGTG20050.018.0790589
Produced by FastQC (version 0.11.5)