FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_C2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_C2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6973891
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG151070.2166222557823172No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA86860.12455026899617445No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT70860.1016075530862183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG133300.025.1817976
TACAGGC83300.024.8918347
TATACAG169100.023.2376145
ACAGGCA93000.022.1396778
TGCAGTC29450.021.9099087
CAGGCAA99800.020.7764519
GAGCGCA22350.020.436149
TACAGGT62100.019.8469227
TAGAGCG24150.019.8135667
GTATATA267650.019.778541
GTATTAG64900.018.883421
TAGGTAG42300.016.9685
CTCTGTG47900.016.8005437
ATTATAC147150.016.7021433
TATATAC310150.016.3161013
TATACCG41700.016.1689855
GTATAAT127250.016.0705221
CACCCGC21550.015.811873145
CAGCGTG16350.015.51989
ATATACA265250.015.4153494
Produced by FastQC (version 0.11.5)