FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_C1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_C1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3930322
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG86800.220847045102157No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA58550.14896998261211167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA18000.027.3884499
TACAGGC51600.026.1332997
TAGAGCG20300.026.0710077
ATACAGG81000.024.8823156
TATACAG99500.023.0247545
ACAGGCA57650.023.0135048
GTATATA151700.021.4633121
CAGGCAA63150.021.1239669
GACTAGG9700.019.4326768
TGCAGTC19950.019.260347
GTATTAG38250.018.0106011
GTATAAT68400.017.4929811
CTAATAC44250.017.366733
TACAGGT36350.017.1524057
ATATACA155300.016.3855534
TCTATAC43600.016.29543
TATACCG26000.016.1728155
TATATAC179000.016.1196463
GGAACTT81400.016.031681145
ATAGAGC35350.015.7918246
Produced by FastQC (version 0.11.5)