FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_B2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_B2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115281
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG239320.4678530856858108No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA152910.2989278594861162No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT123030.24051464621396165No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT105400.2060492864419374No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC96540.1887286348491901No Hit
ATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCT77700.1518978136293979No Hit
GATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTC74690.14601348391222302No Hit
CTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAAGGTT72160.14106751906688997No Hit
GTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAG64150.12540855526802927No Hit
ATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAGCAA63920.12495892210027172No Hit
GTATATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTG61580.12038439335004274No Hit
GGTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGA59800.11690462361696259No Hit
GCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTT58970.11528203435940274No Hit
ATATAGAACAGCTAAAGGGTAGTGCTGAAGGAAGCATACGATACCCCGCA58190.11375719144265976No Hit
ACATAAATAGACGCATATAAGTACGCATTTAAGCATAAACACGCACTATG56520.11049246365937668No Hit
GTATAATAATATATAGTCTAGCGCTTTACGGAAGACAATGTATGTATTTC56380.11021877390508948No Hit
TATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGC53820.10521416125526632No Hit
ATACTAGTATACTCCGTCTACTGTACGATACACTTCCGCTCAGGTCCTTG52210.1020667290809635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG28400.033.6961827
GAGCGCA27600.033.622199
TACAGGC115550.030.8060937
ATACAGG180500.030.4852396
TGCAGTC36150.029.8815447
TATACAG201400.029.661485
ACAGGCA122650.029.022788
TACAGGT68650.027.4573027
TAGGTAG21950.027.4137635
CAGGCAA136150.026.2515149
CTCTGTG49550.026.0436257
ATCTCTG57200.023.8279615
GTATATA245750.023.4301571
TCGAATA74450.023.370237145
GTATTAG47900.023.314891
ATAGAGC44400.023.0229636
GTATAAT73400.022.8225481
TATACCG36100.022.693235
GGAACTT156350.022.534864145
GTAGTCA26850.021.6008458
Produced by FastQC (version 0.11.5)