FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_B1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_B1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3748104
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG84060.22427339262731236No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA52280.13948385637111457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA14200.028.5900789
TAGAGCG15900.026.901137
TAGGTAG20450.024.4608465
ATACAGG80700.023.5365836
TATACAG98500.023.4048865
TACAGGC49850.022.541477
GTATTAG38550.022.1999971
CAGCGTG12850.021.4385879
TACAGGT36100.021.0861727
ACAGGCA55550.020.3589888
GTATATA144550.020.0193561
GTATAAT58800.019.6116941
TGCAGTC19850.018.9914677
ACAGCGT15300.018.9532748
CAGGCAA59800.018.669619
TATACCG22750.017.8452365
TACAGCG18350.017.7783787
ATTATAC71200.017.61523
TCTATAC41250.017.5750893
GTAGTCA24200.017.375148
Produced by FastQC (version 0.11.5)